Search results for " Microtubules"

showing 3 items of 3 documents

Mutant p53 induces Golgi tubulo-vesiculation driving a prometastatic secretome

2020

TP53 missense mutations leading to the expression of mutant p53 oncoproteins are frequent driver events during tumorigenesis. p53 mutants promote tumor growth, metastasis and chemoresistance by affecting fundamental cellular pathways and functions. Here, we demonstrate that p53 mutants modify structure and function of the Golgi apparatus, culminating in the increased release of a pro-malignant secretome by tumor cells and primary fibroblasts from patients with Li-Fraumeni cancer predisposition syndrome. Mechanistically, interacting with the hypoxia responsive factor HIF1α, mutant p53 induces the expression of miR-30d, which in turn causes tubulo-vesiculation of the Golgi apparatus, leading …

0301 basic medicineBiopsyGeneral Physics and AstronomyGolgi ApparatusAnimals Biopsy Breast Neoplasms Cell Line Tumor Cell Transformation Neoplastic Female Fibroblasts Gene Expression Regulation Neoplastic Golgi Apparatus Humans Hypoxia-Inducible Factor 1 alpha Subunit Li-Fraumeni Syndrome Mice MicroRNAs Microtubules Mutation Primary Cell Culture Secretory Vesicles Signal TransductionSkin Tumor Microenvironment Tumor Suppressor Protein p53 Xenograft Model Antitumor Assays02 engineering and technologymedicine.disease_causeCell TransformationMicrotubulesSettore BIO/09 - FisiologiaMetastasisLi-Fraumeni SyndromeMiceTumor MicroenvironmentGolgisecretory machinerySuper-resolution microscopyAnimals; Biopsy; Breast Neoplasms; Cell Line Tumor; Cell Transformation Neoplastic; Female; Fibroblasts; Gene Expression Regulation Neoplastic; Golgi Apparatus; Humans; Hypoxia-Inducible Factor 1 alpha Subunit; Li-Fraumeni Syndrome; Mice; MicroRNAs; Microtubules; Mutation; Primary Cell Culture; Secretory Vesicles; Signal Transduction; Skin; Tumor Microenvironment; Tumor Suppressor Protein p53; Xenograft Model Antitumor Assayslcsh:ScienceSkinMultidisciplinaryTumorChemistrymutant p53QCell migrationMicroRNASecretomics021001 nanoscience & nanotechnologyCell biologyGene Expression Regulation NeoplasticCell Transformation NeoplasticsymbolsFibroblastmiR-30dFemaleHypoxia-Inducible Factor 10210 nano-technologyBreast NeoplasmHumanSignal TransductionCancer microenvironmentStromal cellSecretory VesicleSciencePrimary Cell CultureBreast NeoplasmsMicrotubuleGolgi ApparatuSettore MED/08 - Anatomia Patologicaalpha SubunitGeneral Biochemistry Genetics and Molecular BiologyArticleCell Line03 medical and health sciencessymbols.namesakeCell Line TumormedicineAnimalsHumansSettore MED/05 - Patologia ClinicaSecretionTumor microenvironmentNeoplasticAnimalSecretory VesiclesGeneral ChemistryOncogenesGolgi apparatusHDAC6FibroblastsMicroreviewHypoxia-Inducible Factor 1 alpha SubunitmicroenvironmentXenograft Model Antitumor AssaysMicroRNAs030104 developmental biologyGene Expression RegulationMutationlcsh:QTumor Suppressor Protein p53Carcinogenesis
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Experimental virus evolution reveals a role of plant microtubule dynamics and TORTIFOLIA1/SPIRAL2 in RNA trafficking.

2014

1 tabla y 2 figuras

ArabidopsisPlant ScienceMicrotubulesRNA Transport//purl.org/becyt/ford/1 [https]INFECTIONTobacco mosaic virusTOBACCO-MOSAIC-VIRUSMovement proteinCytoskeletonCytoskeletonGeneticsCoat proteinMultidisciplinaryTRANSGENIC PLANTSQREXPERIMENTAL EVOLUTIONARABIDOPSISBiological Evolution3. Good healthCell biologyMacromolecular assemblyTobacco Mosaic VirusMICROTUBULESMedical MicrobiologyTobamovirusesViral Pathogensdynamic plasticityHost-Pathogen InteractionsMedicineTobacco mosaic viruscortical microtubuleCellular Structures and OrganellesCortical microtubuleARABIDOPSIS CORTICAL MICROTUBULESCell wallsMicrotubule-Associated ProteinsCIENCIAS NATURALES Y EXACTASResearch ArticleEvolutionary ProcessesSciencePlant Cell BiologyPlant PathogensORGANIZATIONBiologyMicrobiologyPlant Viral PathogensCiencias BiológicasMOVEMENT PROTEINComplexesMicrotubuleEvolutionary Adaptation//purl.org/becyt/ford/1.6 [https]Microbial PathogensPlant DiseasesEvolutionary BiologyArabidopsis ProteinsBotánicaRNABiology and Life SciencesCell BiologyPlant PathologyTMVCytoplasmMutationRNAVirologíaHELICAL GROWTHPloS one
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Synthesis, cytotoxicity, and inhibitory effects on tubulin polymerization of a new 3-heterocyclo substituted 2-styrylquinazolinones

2004

In order to study the influence of 3-substitution on the cytotoxic activity of 2-styrylquinazolinones, new 6-chloro-2-styryl-3-(heteroaryl)-4(3H)-quinazolinones were synthesized by refluxing equimolar amounts of 6-chloro-2-methyl-3-(heteroaryl)-4(3H)-quinazolinones and benzaldehyde in glacial acetic acid. At 1 microg ml(-1) concentration, almost all 2-styrylquinazolinones showed some cytotoxic activity against the L1210 and K562 leukemia cell lines. However, only 6-chloro-2-styryl-3-(pyrimidin-2yl)-4(3H)-quinazolinone inhibited the growth of these cells by over 50%. This last compound was also the only member of the series that inhibited tubulin polymerization, with an IC(50) value of 5.8 v…

Mitotic indexCell SurvivalPolymersAntineoplastic AgentsSettore BIO/19 - Microbiologia GeneraleMicrotubuleschemistry.chemical_compoundAcetic acidHeterocyclic CompoundsTubulinMicrotubuleDrug DiscoveryTumor Cells CulturedmedicineColchicineAnimalsHumansCytotoxic T cellCytotoxicityPharmacologyMolecular StructureChemistryTubulin ModulatorsOrganic ChemistryBiological activityGeneral MedicineMolecular biologySettore CHIM/08 - Chimica FarmaceuticaTubulin ModulatorsRatsMechanism of actionBiochemistryCell cultureQuinazolinesDrug Screening Assays Antitumormedicine.symptomK562 cells2-Styrylquinazolinones Antimitotic agents Cytotoxic activity MicrotubulesEuropean Journal of Medicinal Chemistry
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